Background Membrane Imaging
Method Introduction
Backgrounded membrane imaging (BMI) is an automated variation of the light microscopy compendial method. It allows the sizing, quantification, and morphological characterization of micrometer-sized particles.
For BMI analysis, liquid samples are applied onto a 96-well filter plate, and the suspended particles are then filtered and dried on the filter membrane. Subsequently, a microscope camera captures bright-field images of the respective filter area. The digital images of the particles on the filter are processed by background subtraction algorithms and image morphology analysis software that allows their quantification in size and count.
Need more information? Follow the links below and contact our experts with your questions today.
Applications
BMI is currently used as a research tool to support more established particle characterization and quantification techniques, such as flow imaging microscopy (FIM) and light obscuration (LO). BMI’s benefits include the low sample volume, the short measurement times owing to the well plate set-up, and the supposedly higher image contrast than particles in suspension.
Quality and Biosafety Level
We provide all our analytical services with the highest quality standards. Experienced scientists carry out each project, and a scientific reviewer comprehensively checks every report or data presentation.
We offer this technology with the following quality and biosafety levels:
R&D level
We offer this method under R&D. Our GRP system assures the highest-quality research standards.
Up to biosafety level 1
This method can be applied to proteins, nucleic acids, most viral vectors, including AAVs and more.
Analytical Method Development, Qualification and Validation
For common sample types, we can often apply standardized methods with little setup effort. However, when needed, our experienced analytical experts create or optimize custom methods tailored to your active pharmaceutical ingredient, product type and development phase.
Talk to Our Experts or Request a Quote
Our expert team is ready to answer your questions and guide you to the services best suited to your program’s modality, stage and challenge. If your needs are well-defined, we’ll begin the quotation process.